What is the name of C6H5COONa

Table 1 Inorganic Carbon Values ​​on Each Analysis Occasion

 

 

 

Day

Inorganic Carbon (mg IC)

Inoculum Control

Procedure control

Test item

Toxicity Control

R.1

R2

R1

R2

R1

R2

R1

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0

2.10

2.10

2.10

2.10

2.10

2.10

2.10

2.10

2.10

2.22

2.10

2.10

2.10

2.22

2

7.42

-

6.96

-

22.16

-

25.29

-

11.72

-

9.86

-

27.38

-

6

14.65

-

14.65

-

32.76

-

32.29

-

24.68

-

18.11

-

47.06

-

8

19.03

-

17.54

-

39.10

-

44.38

-

23.97

-

24.19

-

60.77

-

10

19.61

-

18.24

-

43.66

-

41.15

-

25.42

-

27.59

-

55.63

-

14

24.82

-

21.31

-

43.41

-

44.88

-

29.24

-

28.79

-

59.73

-

21

30.76

-

27.27

-

50.93

-

56.90

-

34.25*

-

34.59*

-

69.74

-

28

34.83

-

30.80

-

52.64

-

60.14

-

39.65

-

37.41

-

64.18

-

R1– R2 = Replicates 1 and 2 Abs = CO2 absorber vessels

* = Results from analysis of frozen sample

Table 2 Percentage Biodegradation Values

 

Day

% Biodegradation

Procedure control

Test item

Toxicity Control

0

0

0

0

2

55

12

34

6

60

23

54

8

78

19

71

10

78

25

61

14

70

20

61

21

83

18

68

28

79

19

52

Table 3 Total and Inorganic Carbon Values ​​in the Culture Vessels on Day 0

 

Test vessel

Total carbon *

(mg / L)

Inorganic Carbon * (mg / L)

 

IC content (% of TC)

Test item

10 mg C / L R1

 

10.08**

 

0.19

 

2

Test item

10 mg C / L R2

 

10.10**

 

0.14

 

1

R1– R2 = Replicates 1 and 2

* Corrected for control values

** Total carbon value given is the sum of the TC value obtained from analysis and the nominal TC contribution of the test item

Table 4 pH Values ​​of the Test Preparations on Days 0 and 28

 

 

Test vessel

 

pH

Day 0

Pre-adjustment

Day 0

Post adjustment

 

Day 28

Inoculum Control R1

7.7

7.6

7.6

Inoculum Control R2

7.7

7.5

7.5

Procedure control R1

7.7

7.5

7.6

Procedure Control R2

7.7

7.5

7.6

Test Item R1

7.7

7.5

7.5

Test item R2

7.7

7.5

7.5

Toxicity Control

7.7

7.5

7.6

R1 – R2 = Replicates 1 and 2

Table 5 Observations on the Test Preparations Throughout the Test Period

 

Test vessel

Observations on Test Preparations

Day 0

Day 6

Day 13

Day 20

Day 27

Inoculum Control

 

R1

Light brown dispersion

Light brown dispersion

Light brown dispersion

Light brown dispersion

Light brown dispersion

 

R2

Light brown dispersion

Light brown dispersion

Light brown dispersion

Light brown dispersion

Light brown dispersion

 

Procedure control

 

R1

Light brown dispersion, no undissolved reference item visible

Light brown dispersion, no undissolved reference item visible

Light brown dispersion, no undissolved reference item visible

Light brown dispersion, no undissolved reference item visible

Light brown dispersion, no undissolved reference item visible

 

 

R2

Light brown dispersion, no undissolved reference item visible

Light brown dispersion, no undissolved reference item visible

Light brown dispersion, no undissolved reference item visible

Light brown dispersion, no undissolved reference item visible

Light brown dispersion, no undissolved reference item visible

 

 

 

Test item

 

 

R1

Light brown dispersion with few particles of test item visible dispersed throughout

Light brown dispersion with few particles of test item visible dispersed throughout

Light brown dispersion with few particles of test item visible dispersed throughout

Light brown dispersion with few particles of test item visible dispersed throughout

Light brown dispersion with few particles of test item visible dispersed throughout

 

 

R2

Light brown dispersion with few particles of test item visible dispersed throughout

Light brown dispersion with few particles of test item visible dispersed throughout

Light brown dispersion with few particles of test item visible dispersed throughout

Light brown dispersion with few particles of test item visible dispersed throughout

Light brown dispersion with few particles of test item visible dispersed throughout

 

 

 

 

Toxicity Control

 

Light brown dispersion with few particles of test item visible dispersed throughout.

No undissloved reference item visible

Light brown dispersion with few particles of test item visible dispersed throughout.

No undissloved reference item visible

Light brown dispersion with few particles of test item visible dispersed throughout.

No undissloved reference item visible

Light brown dispersion with few particles of test item visible dispersed throughout.

No undissloved reference

item visible

Light brown dispersion with few particles of test item visible dispersed throughout.

No undissloved reference

item visible

R1– R2 = Replicates 1 and 2

Introduction

A study was performed to assess the ready biodegradability of the test item in an aerobic aqueous medium. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No. 301B, "Ready Biodegradability; CO2 Evolution Test" referenced as Method C.4-C of Commission Regulation (EC) No. 440/2008 and US EPA Fate, Transport, and Transformation Test Guidelines OCSPP 835.3110 (Paragraph (m)).

 

Methods

The test item, at a concentration of 10 mg carbon / L, was exposed to activated sewage sludge micro-organisms with mineral medium in sealed culture vessels in the dark at temperatures of between 22 and 24OC for 28 days.

The biodegradation of the test item was assessed by the determination of carbon dioxide produced. Control solutions with inoculum and the reference item, sodium benzoate, together with a toxicity control were used for validation purposes.

 

Results

The test item attained 19% biodegradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301B.