# What is the name of C6H5COONa

Table 1 Inorganic Carbon Values ​​on Each Analysis Occasion

 Day Inorganic Carbon (mg IC) Inoculum Control Procedure control Test item Toxicity Control R.1 R2 R1 R2 R1 R2 R1 Section1 Paragraph 2 Paragraph 1 Paragraph 2 Paragraph 1 Paragraph 2 Paragraph 1 Paragraph 2 Paragraph 1 Paragraph 2 Paragraph 1 Paragraph 2 Paragraph 1 Paragraph 2 0 2.10 2.10 2.10 2.10 2.10 2.10 2.10 2.10 2.10 2.22 2.10 2.10 2.10 2.22 2 7.42 - 6.96 - 22.16 - 25.29 - 11.72 - 9.86 - 27.38 - 6 14.65 - 14.65 - 32.76 - 32.29 - 24.68 - 18.11 - 47.06 - 8 19.03 - 17.54 - 39.10 - 44.38 - 23.97 - 24.19 - 60.77 - 10 19.61 - 18.24 - 43.66 - 41.15 - 25.42 - 27.59 - 55.63 - 14 24.82 - 21.31 - 43.41 - 44.88 - 29.24 - 28.79 - 59.73 - 21 30.76 - 27.27 - 50.93 - 56.90 - 34.25* - 34.59* - 69.74 - 28 34.83 - 30.80 - 52.64 - 60.14 - 39.65 - 37.41 - 64.18 -

R1– R2 = Replicates 1 and 2 Abs = CO2 absorber vessels

* = Results from analysis of frozen sample

 Day % Biodegradation Procedure control Test item Toxicity Control 0 0 0 0 2 55 12 34 6 60 23 54 8 78 19 71 10 78 25 61 14 70 20 61 21 83 18 68 28 79 19 52

Table 3 Total and Inorganic Carbon Values ​​in the Culture Vessels on Day 0

 Test vessel Total carbon *(mg / L) Inorganic Carbon * (mg / L) IC content (% of TC) Test item10 mg C / L R1 10.08** 0.19 2 Test item10 mg C / L R2 10.10** 0.14 1

R1– R2 = Replicates 1 and 2

* Corrected for control values

** Total carbon value given is the sum of the TC value obtained from analysis and the nominal TC contribution of the test item

Table 4 pH Values ​​of the Test Preparations on Days 0 and 28

 Test vessel pH Day 0Pre-adjustment Day 0Post adjustment Day 28 Inoculum Control R1 7.7 7.6 7.6 Inoculum Control R2 7.7 7.5 7.5 Procedure control R1 7.7 7.5 7.6 Procedure Control R2 7.7 7.5 7.6 Test Item R1 7.7 7.5 7.5 Test item R2 7.7 7.5 7.5 Toxicity Control 7.7 7.5 7.6

R1 – R2 = Replicates 1 and 2

Table 5 Observations on the Test Preparations Throughout the Test Period

 Test vessel Observations on Test Preparations Day 0 Day 6 Day 13 Day 20 Day 27 Inoculum Control R1 Light brown dispersion Light brown dispersion Light brown dispersion Light brown dispersion Light brown dispersion R2 Light brown dispersion Light brown dispersion Light brown dispersion Light brown dispersion Light brown dispersion Procedure control R1 Light brown dispersion, no undissolved reference item visible Light brown dispersion, no undissolved reference item visible Light brown dispersion, no undissolved reference item visible Light brown dispersion, no undissolved reference item visible Light brown dispersion, no undissolved reference item visible R2 Light brown dispersion, no undissolved reference item visible Light brown dispersion, no undissolved reference item visible Light brown dispersion, no undissolved reference item visible Light brown dispersion, no undissolved reference item visible Light brown dispersion, no undissolved reference item visible Test item R1 Light brown dispersion with few particles of test item visible dispersed throughout Light brown dispersion with few particles of test item visible dispersed throughout Light brown dispersion with few particles of test item visible dispersed throughout Light brown dispersion with few particles of test item visible dispersed throughout Light brown dispersion with few particles of test item visible dispersed throughout R2 Light brown dispersion with few particles of test item visible dispersed throughout Light brown dispersion with few particles of test item visible dispersed throughout Light brown dispersion with few particles of test item visible dispersed throughout Light brown dispersion with few particles of test item visible dispersed throughout Light brown dispersion with few particles of test item visible dispersed throughout Toxicity Control Light brown dispersion with few particles of test item visible dispersed throughout.No undissloved reference item visible Light brown dispersion with few particles of test item visible dispersed throughout.No undissloved reference item visible Light brown dispersion with few particles of test item visible dispersed throughout.No undissloved reference item visible Light brown dispersion with few particles of test item visible dispersed throughout.No undissloved reference item visible Light brown dispersion with few particles of test item visible dispersed throughout.No undissloved reference item visible

R1– R2 = Replicates 1 and 2

Introduction

A study was performed to assess the ready biodegradability of the test item in an aerobic aqueous medium. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No. 301B, "Ready Biodegradability; CO2 Evolution Test" referenced as Method C.4-C of Commission Regulation (EC) No. 440/2008 and US EPA Fate, Transport, and Transformation Test Guidelines OCSPP 835.3110 (Paragraph (m)).

Methods

The test item, at a concentration of 10 mg carbon / L, was exposed to activated sewage sludge micro-organisms with mineral medium in sealed culture vessels in the dark at temperatures of between 22 and 24OC for 28 days.

The biodegradation of the test item was assessed by the determination of carbon dioxide produced. Control solutions with inoculum and the reference item, sodium benzoate, together with a toxicity control were used for validation purposes.

Results

The test item attained 19% biodegradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301B.